Advances in stimulated Raman scattering imaging for tissues and animals
نویسندگان
چکیده
منابع مشابه
Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering
Brain is an immensely complex system displaying dynamic and heterogeneous metabolic activities. Visualizing cellular metabolism of nucleic acids, proteins, and lipids in brain with chemical specificity has been a long-standing challenge. Recent development in metabolic labeling of small biomolecules allows the study of these metabolisms at the global level. However, these techniques generally r...
متن کاملFast Vibrational Imaging of Single Cells and Tissues by Stimulated Raman Scattering Microscopy
Traditionally, molecules are analyzed in a test tube. Taking biochemistry as an example, the majority of our knowledge about cellular content comes from analysis of fixed cells or tissue homogenates using tools such as immunoblotting and liquid chromatography-mass spectrometry. These tools can indicate the presence of molecules but do not provide information on their location or interaction wit...
متن کاملLabel-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy.
A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.
متن کاملVideo-rate molecular imaging in vivo with stimulated Raman scattering.
Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not...
متن کاملSideward Stimulated Raman Scattering
The SRS of a short laser pulse is important in the contexts of particle acceleration2 and inertial confinement fusion.3,4 In previous studies of the spatiotemporal evolution of SRS,5–13 the Stokes waves were allowed to pass freely through the pulse boundaries. However, the radial ponderomotive force associated with the pulse can expel plasma from the neighborhood of the pulse axis, in which cas...
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ژورنال
عنوان ژورنال: Quantitative Imaging in Medicine and Surgery
سال: 2020
ISSN: 2223-4292,2223-4306
DOI: 10.21037/qims-20-712